Speaker:
Dr Russ Grant. VP Research and Development. Labcorp.
Dr Grant received a PhD in chromatographic and mass spectrometric techniques from Swansea University in 1995. In the intervening years, Dr Grant has worked in the manufacturing, CRO, pharmaceutical and diagnostic arenas, employing mass spectrometric techniques and technologies in drug development, biomarker analysis and in the direct care of patients in clinical medicine.
Overview:
This talk aims to either support or debunk the often used term “Mass spectrometry is accurate, fast and specific” through my personal experience over the last 30 years. This lecture will highlight the achilles heel(s) of quantitative mass spectrometry, together with practically applicable techniques to mitigate and correct them. While it is true that Mass Spectrometry is a powerful technology, one must remember the words of Ben Parker to Spiderman “With great power comes great responsibility”
Objectives:
At the end of this activity, participants will be able to:
- Understand why matrix effects occur (preparatively and through ionization) and know what techniques to use to diagnose and recommendations on how to solve for them.
- How to apply multiple transition ratio monitoring for SRM/MRM to add confidence in analytical selectivity prior to accepting results
- How to improve throughput – without sacrificing selectivity
- The critical importance of metrology in assay development/design
- How to quantify a protein using bottom-up proteomic techniques – “correctly”