speaker:
Dr. Eleonora Petryayeva joined Shared Health as Clinical Biochemist in 2021. Her responsibilities physician consultation and include oversight in areas related to endocrinology, trace elements, biogenic amines, lipids, vitamins and liver tests. She is also involved in multiple projects focusing on improving quality assurance, implementation of new technologies, and expanding mass spectrometry-based testing at Shared Health laboratories. She is the Director for Clinical Biochemistry Post-PhD Residency Program. Dr. Petryayeva completed Hon.B.Sc. and M.Sc. at the University of Toronto in Forensic Chemistry and Bioanalytical Chemistry, respectively. She obtained her PhD in Bioanalytical Chemistry from the University of British Columbia. Then, Dr. Petryayeva completed Clinical Biochemistry Fellowship program at the McMaster University and became certified Clinical Biochemist and a Fellow of the Canadian Academy of Clinical Biochemistry in 2022.
OVERVIEW:
This presentation will provide a brief overview of mass spectrometry techniques used in clinical laboratories. Current standards and best practices for method development and validation of liquid chromatography tandem mass spectrometry (LC-MS/MS) assays in clinical laboratory will be discussed. Examples from our two-year journey to transfer and redevelop assays on new LC-MS/MS instruments will be provided to highlight challenges and troubleshooting approaches. In addition, our experience with improving workflow by integrating automated liquid handler and lab information system (LIS) connectivity will be presented.
learning objectives:
At the conclusion of this session, participants will be able to:
1) Describe the principle of mass spectrometry and recognize common approaches for qualitative and quantitative analysis
2) Describe common uses, advantages and limitations of LC-MS/MS testing
3) Describe current recommendations for method development and validations, focusing on parameters unique to mass spectrometry
4) Recognize common bottlenecks in mass spectrometry testing and strategies to improve workflow in clinical laboratory